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Title : 7.344 Directed Evolution: Engineering Biocatalysts (MIT)

Title : 7.344 Directed Evolution: Engineering Biocatalysts (MIT)

Description : Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current

Description : Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current

Fromsemester : Spring

Fromsemester : Spring

Fromyear : 2008

Fromyear : 2008

Creator :

Creator :

Date : 2008-12-17T14:41:10+05:00

Date : 2008-12-17T14:41:10+05:00

Relation : 7.344

Relation : 7.344

Language : en-US

Language : en-US

Subject : evolution

Subject : evolution

Subject : biocatalyst

Subject : biocatalyst

Subject : mutation

Subject : mutation

Subject : library

Subject : library

Subject : recombination

Subject : recombination

Subject : directed evolution

Subject : directed evolution

Subject : enzyme

Subject : enzyme

Subject : point mutation

Subject : point mutation

Subject : mutagenesis

Subject : mutagenesis

Subject : DNA

Subject : DNA

Subject : gene

Subject : gene

Subject : complementation

Subject : complementation

Subject : affinity

Subject : affinity

Subject : phage

Subject : phage

Subject : ribosome display

Subject : ribosome display

Subject : yeast surface display

Subject : yeast surface display

Subject : bacterial cell surface display

Subject : bacterial cell surface display

Subject : IVC

Subject : IVC

Subject : FACS

Subject : FACS

Subject : active site

Subject : active site