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7.60 Cell Biology: Structure and Functions of the Nucleus (MIT) 7.60 Cell Biology: Structure and Functions of the Nucleus (MIT)

Description

This course covers the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Topics include Eukaryotic genome structure, function, and expression, processing of RNA, and regulation of the cell cycle. The techniques and logic used to address important problems in nuclear cell biology is emphasized. Lectures cover broad topic areas in nuclear cell biology and class discussions focus on representative papers recently published in the field. This course covers the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Topics include Eukaryotic genome structure, function, and expression, processing of RNA, and regulation of the cell cycle. The techniques and logic used to address important problems in nuclear cell biology is emphasized. Lectures cover broad topic areas in nuclear cell biology and class discussions focus on representative papers recently published in the field.

Subjects

cell biology | cell biology | nucleus | nucleus | biology | biology | nuclear cell biology | nuclear cell biology | DNA replication | DNA replication | DNA repair | DNA repair | DNA | DNA | genome | genome | cell cycle control | cell cycle control | chromatin | chromatin | gene expression | gene expression | replication | replication | transcription | transcription | RNA | RNA | RNA interference | RNA interference | mRNA | mRNA | microRNA | microRNA | RNAi | RNAi

License

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7.16 Experimental Molecular Biology: Biotechnology II (MIT) 7.16 Experimental Molecular Biology: Biotechnology II (MIT)

Description

The course applies molecular biology and reverse genetics approaches to the study of apoptosis, or programmed cell death (PCD), in Drosophila cells. RNA interference (RNAi), or double stranded RNA-mediated gene silencing, will be used to inhibit expression of candidate apoptosis-related genes in cultured Drosophila cells. Teams of 2 or 3 students will design and carry out experiments to address questions about the genes involved in the regulation and execution of PCD in this system. Some projects involve the use of DNA damaging agents or other cytotoxic chemicals or drugs to help understand the pathways that control a cell's decision to undergo apoptosis. Instruction and practice in written and oral communication are provided. The course applies molecular biology and reverse genetics approaches to the study of apoptosis, or programmed cell death (PCD), in Drosophila cells. RNA interference (RNAi), or double stranded RNA-mediated gene silencing, will be used to inhibit expression of candidate apoptosis-related genes in cultured Drosophila cells. Teams of 2 or 3 students will design and carry out experiments to address questions about the genes involved in the regulation and execution of PCD in this system. Some projects involve the use of DNA damaging agents or other cytotoxic chemicals or drugs to help understand the pathways that control a cell's decision to undergo apoptosis. Instruction and practice in written and oral communication are provided.

Subjects

RNAi | RNAi | RNA interference | RNA interference | programmed cell death | programmed cell death | Drosophilia | Drosophilia | PCD | PCD | mRNA | mRNA | lab notebook | lab notebook | scientific writing | scientific writing | RT-PCR | RT-PCR | S2 RNA | S2 RNA | S2 | S2 | cell culture | cell culture | genetic transcription | genetic transcription | dsRNA | dsRNA | bioinformatics | bioinformatics

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.345 Non-coding RNAs: Junk or Critical Regulators in Health and Disease? (MIT) 7.345 Non-coding RNAs: Junk or Critical Regulators in Health and Disease? (MIT)

Description

Every time we scientists think that we have dissected the precise biological nature of a process, an incidental finding, a brilliantly designed experiment, or an unexpected result can turn our world upside down. Until recently thought by many to be cellular "junk" because they do not encode proteins, non-coding RNAs are gaining a growing recognition for their roles in the regulation of a wide scope of processes, ranging from embryogenesis and development to cancer and degenerative disorders. The aim of this class is to introduce the diversity of the RNA world, inhabited by microRNAs, lincRNAs, piRNAs, and many others. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in us Every time we scientists think that we have dissected the precise biological nature of a process, an incidental finding, a brilliantly designed experiment, or an unexpected result can turn our world upside down. Until recently thought by many to be cellular "junk" because they do not encode proteins, non-coding RNAs are gaining a growing recognition for their roles in the regulation of a wide scope of processes, ranging from embryogenesis and development to cancer and degenerative disorders. The aim of this class is to introduce the diversity of the RNA world, inhabited by microRNAs, lincRNAs, piRNAs, and many others. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in us

Subjects

Non-coding RNAs | Non-coding RNAs | microRNAs | microRNAs | lincRNAs | lincRNAs | piRNAs | piRNAs | RNA interference | RNA interference | miRNA | miRNA | tumor suppressors and oncogenes | tumor suppressors and oncogenes | RNAi therapeutics | RNAi therapeutics

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.349 Stem Cells: A Cure or Disease? (MIT) 7.349 Stem Cells: A Cure or Disease? (MIT)

Description

Have you ever considered going to a pharmacy to order some new cardiomyocytes (heart muscle cells) for your ailing heart? It might sound crazy, but recent developments in stem cell science have made this concept not so futuristic. In this course, we will explore the underlying biology behind the idea of using stem cells to treat disease, specifically analyzing the mechanisms that enable a single genome to encode multiple cell states ranging from neurons to fibroblasts to T cells. Overall, we hope to provide a comprehensive overview of this exciting new field of research and its clinical relevance. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literat Have you ever considered going to a pharmacy to order some new cardiomyocytes (heart muscle cells) for your ailing heart? It might sound crazy, but recent developments in stem cell science have made this concept not so futuristic. In this course, we will explore the underlying biology behind the idea of using stem cells to treat disease, specifically analyzing the mechanisms that enable a single genome to encode multiple cell states ranging from neurons to fibroblasts to T cells. Overall, we hope to provide a comprehensive overview of this exciting new field of research and its clinical relevance. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literat

Subjects

stem cells | stem cells | stem cell therapy | stem cell therapy | cellular reprogramming | cellular reprogramming | transdifferentiation | transdifferentiation | pluripotency | pluripotency | epigenetics | epigenetics | genome-wide sequencing | genome-wide sequencing | transcription-mediated reprogramming | transcription-mediated reprogramming | embryonic stem cell technology | embryonic stem cell technology | transcription factors | transcription factors | chromatin structure | chromatin structure | H3K4me3 | H3K4me3 | H3K27me3 | H3K27me3 | histone deacetylase 1 | histone deacetylase 1 | RNAi screens | RNAi screens | Oct4 | Oct4 | cloning | cloning | Dolly | Dolly | in vitro differentiation | in vitro differentiation | regenerative medicine | regenerative medicine

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.60 Cell Biology: Structure and Functions of the Nucleus (MIT) 7.60 Cell Biology: Structure and Functions of the Nucleus (MIT)

Description

The goal of this course is to teach both the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Lectures and class discussions will cover the background and fundamental findings in a particular area of nuclear cell biology. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish these findings. Some examples of topics include genome and systems biology, transcription, and gene expression. The goal of this course is to teach both the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Lectures and class discussions will cover the background and fundamental findings in a particular area of nuclear cell biology. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish these findings. Some examples of topics include genome and systems biology, transcription, and gene expression.

Subjects

cell biology | cell biology | nucleus | nucleus | biology | biology | nuclear cell biology | nuclear cell biology | DNA replication | DNA replication | DNA repair | DNA repair | DNA | DNA | genome | genome | cell cycle control | cell cycle control | transcriptional regulation | transcriptional regulation | gene expression | gene expression | chromatin | chromatin | chromosomes | chromosomes | replication | replication | transcription | transcription | RNA | RNA | RNA interference | RNA interference | mRNA | mRNA | microRNA | microRNA | RNAi | RNAi

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.346 RNAi: A Revolution in Biology and Therapeutics (MIT) 7.346 RNAi: A Revolution in Biology and Therapeutics (MIT)

Description

Despite centuries of effort, modern medicine still struggles to find the source of disease and to provide specific treatment without side effects. Both traditional small molecules and protein-based therapeutics have achieved only limited success. What is the next therapeutic frontier? The answer may be RNA interference. In this course, we will focus on the therapeutic potential of RNAi. We will discuss its discovery functions in normal biological processes, utility as an experimental tool, potential for therapeutic use, and pursuit by the biotechnology industry. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current Despite centuries of effort, modern medicine still struggles to find the source of disease and to provide specific treatment without side effects. Both traditional small molecules and protein-based therapeutics have achieved only limited success. What is the next therapeutic frontier? The answer may be RNA interference. In this course, we will focus on the therapeutic potential of RNAi. We will discuss its discovery functions in normal biological processes, utility as an experimental tool, potential for therapeutic use, and pursuit by the biotechnology industry. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current

Subjects

RNAi | RNAi | RNA interface | RNA interface | therapeutics | therapeutics | siRNA | siRNA | miRNA | miRNA | shRNA | shRNA

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.344 RNA Interference: A New Tool for Genetic Analysis and Therapeutics (MIT) 7.344 RNA Interference: A New Tool for Genetic Analysis and Therapeutics (MIT)

Description

This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. To understand and treat any disease with a genetic basis or predisposition, scientists and clinicians need effective ways of manipulating the levels of genes and gene products. Conventional methods for the genetic modification of many experimental organisms are technically demanding and time consuming. Just over 5 years ago, a new mechanism of gene-silencing, termed RNA interference (RNAi), was discovered. In addition to being a fascinating biological process, RNAi provides a revolutionary technology that has a This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. To understand and treat any disease with a genetic basis or predisposition, scientists and clinicians need effective ways of manipulating the levels of genes and gene products. Conventional methods for the genetic modification of many experimental organisms are technically demanding and time consuming. Just over 5 years ago, a new mechanism of gene-silencing, termed RNA interference (RNAi), was discovered. In addition to being a fascinating biological process, RNAi provides a revolutionary technology that has a

Subjects

RNA interference | RNA interference | RNAi | RNAi | RNA | RNA | genetic analysis | genetic analysis | gene therapy | gene therapy | gene products | gene products | gene silencing | gene silencing | gene expression | gene expression | human disease models | human disease models | mRNA | mRNA | genetic interference | genetic interference | short interfering RNA | short interfering RNA | siRNAs | siRNAs | expression vectors | expression vectors | RNA sequences | RNA sequences | nucleotide fragments | nucleotide fragments | microRNA | microRNA | mRNA degradation | mRNA degradation | transgenic mice | transgenic mice | lentivirus | lentivirus | knock-down animals | knock-down animals | tissue specificity | tissue specificity

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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HST.161 Molecular Biology and Genetics in Modern Medicine (MIT) HST.161 Molecular Biology and Genetics in Modern Medicine (MIT)

Description

This course provides a foundation for understanding the relationship between molecular biology, developmental biology, genetics, genomics, bioinformatics, and medicine. It develops explicit connections between basic research, medical understanding, and the perspective of patients. Principles of human genetics are reviewed. We translate clinical understanding into analysis at the level of the gene, chromosome and molecule; we cover the concepts and techniques of molecular biology and genomics, and the strategies and methods of genetic analysis, including an introduction to bioinformatics. Material in the course extends beyond basic principles to current research activity in human genetics. This course provides a foundation for understanding the relationship between molecular biology, developmental biology, genetics, genomics, bioinformatics, and medicine. It develops explicit connections between basic research, medical understanding, and the perspective of patients. Principles of human genetics are reviewed. We translate clinical understanding into analysis at the level of the gene, chromosome and molecule; we cover the concepts and techniques of molecular biology and genomics, and the strategies and methods of genetic analysis, including an introduction to bioinformatics. Material in the course extends beyond basic principles to current research activity in human genetics.

Subjects

Genetics | Genetics | genes | genes | genetic disorders | genetic disorders | inborn error | inborn error | muscular dystrophy | muscular dystrophy | PKU | PKU | phenylketoneuria | phenylketoneuria | cancer | cancer | tumors | tumors | gene therapy | gene therapy | disease | disease | birth defects | birth defects | chromosomes | chromosomes | leukemia | leukemia | RNAi | RNAi | hemophilia | hemophilia | thalassemia | thalassemia | deafness | deafness | mutations | mutations | hypertrophic cardiomyopathy | hypertrophic cardiomyopathy | epigenetics | epigenetics | rett syndrome | rett syndrome | prenatal diagnosis | prenatal diagnosis | LOD scores | LOD scores | gene linkage | gene linkage | mitochondrial disorders | mitochondrial disorders | degenerative disorders | degenerative disorders | complex traits | complex traits | Mendelian inheritance | Mendelian inheritance

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.15 Experimental Molecular Genetics (MIT) 7.15 Experimental Molecular Genetics (MIT)

Description

This project-based laboratory course provides students with in-depth experience in experimental molecular genetics, using modern methods of molecular biology and genetics to conduct original research. The course is geared towards students (including sophomores) who have a strong interest in a future career in biomedical research. This semester will focus on chemical genetics using Caenorhabditis elegans as a model system. Students will gain experience in research rationale and methods, as well as training in the planning, execution, and communication of experimental biology. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear This project-based laboratory course provides students with in-depth experience in experimental molecular genetics, using modern methods of molecular biology and genetics to conduct original research. The course is geared towards students (including sophomores) who have a strong interest in a future career in biomedical research. This semester will focus on chemical genetics using Caenorhabditis elegans as a model system. Students will gain experience in research rationale and methods, as well as training in the planning, execution, and communication of experimental biology. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear

Subjects

molecular genetics | molecular genetics | molecular biology | molecular biology | chemical genetics | chemical genetics | Caenorhabditis elegans | Caenorhabditis elegans | experimental biology | experimental biology | bioinformatics | bioinformatics | genetic linkage | genetic linkage | SNP mapping | SNP mapping | RNAi | RNAi | Gibson assembly | Gibson assembly | cDNA | cDNA | PCR | PCR | Primer design | Primer design | RNA extraction | RNA extraction | chemotaxis assay | chemotaxis assay | Next Generation Sequencing | Next Generation Sequencing

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.60 Cell Biology: Structure and Functions of the Nucleus (MIT)

Description

This course covers the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Topics include Eukaryotic genome structure, function, and expression, processing of RNA, and regulation of the cell cycle. The techniques and logic used to address important problems in nuclear cell biology is emphasized. Lectures cover broad topic areas in nuclear cell biology and class discussions focus on representative papers recently published in the field.

Subjects

cell biology | nucleus | biology | nuclear cell biology | DNA replication | DNA repair | DNA | genome | cell cycle control | chromatin | gene expression | replication | transcription | RNA | RNA interference | mRNA | microRNA | RNAi

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.15 Experimental Molecular Genetics (MIT)

Description

This project-based laboratory course provides students with in-depth experience in experimental molecular genetics, using modern methods of molecular biology and genetics to conduct original research. The course is geared towards students (including sophomores) who have a strong interest in a future career in biomedical research. This semester will focus on chemical genetics using Caenorhabditis elegans as a model system. Students will gain experience in research rationale and methods, as well as training in the planning, execution, and communication of experimental biology. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear

Subjects

molecular genetics | molecular biology | chemical genetics | Caenorhabditis elegans | experimental biology | bioinformatics | genetic linkage | SNP mapping | RNAi | Gibson assembly | cDNA | PCR | Primer design | RNA extraction | chemotaxis assay | Next Generation Sequencing

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.345 Non-coding RNAs: Junk or Critical Regulators in Health and Disease? (MIT)

Description

Every time we scientists think that we have dissected the precise biological nature of a process, an incidental finding, a brilliantly designed experiment, or an unexpected result can turn our world upside down. Until recently thought by many to be cellular "junk" because they do not encode proteins, non-coding RNAs are gaining a growing recognition for their roles in the regulation of a wide scope of processes, ranging from embryogenesis and development to cancer and degenerative disorders. The aim of this class is to introduce the diversity of the RNA world, inhabited by microRNAs, lincRNAs, piRNAs, and many others. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in us

Subjects

Non-coding RNAs | microRNAs | lincRNAs | piRNAs | RNA interference | miRNA | tumor suppressors and oncogenes | RNAi therapeutics

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.349 Stem Cells: A Cure or Disease? (MIT)

Description

Have you ever considered going to a pharmacy to order some new cardiomyocytes (heart muscle cells) for your ailing heart? It might sound crazy, but recent developments in stem cell science have made this concept not so futuristic. In this course, we will explore the underlying biology behind the idea of using stem cells to treat disease, specifically analyzing the mechanisms that enable a single genome to encode multiple cell states ranging from neurons to fibroblasts to T cells. Overall, we hope to provide a comprehensive overview of this exciting new field of research and its clinical relevance. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literat

Subjects

stem cells | stem cell therapy | cellular reprogramming | transdifferentiation | pluripotency | epigenetics | genome-wide sequencing | transcription-mediated reprogramming | embryonic stem cell technology | transcription factors | chromatin structure | H3K4me3 | H3K27me3 | histone deacetylase 1 | RNAi screens | Oct4 | cloning | Dolly | in vitro differentiation | regenerative medicine

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.60 Cell Biology: Structure and Functions of the Nucleus (MIT)

Description

The goal of this course is to teach both the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Lectures and class discussions will cover the background and fundamental findings in a particular area of nuclear cell biology. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish these findings. Some examples of topics include genome and systems biology, transcription, and gene expression.

Subjects

cell biology | nucleus | biology | nuclear cell biology | DNA replication | DNA repair | DNA | genome | cell cycle control | transcriptional regulation | gene expression | chromatin | chromosomes | replication | transcription | RNA | RNA interference | mRNA | microRNA | RNAi

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.346 RNAi: A Revolution in Biology and Therapeutics (MIT)

Description

Despite centuries of effort, modern medicine still struggles to find the source of disease and to provide specific treatment without side effects. Both traditional small molecules and protein-based therapeutics have achieved only limited success. What is the next therapeutic frontier? The answer may be RNA interference. In this course, we will focus on the therapeutic potential of RNAi. We will discuss its discovery functions in normal biological processes, utility as an experimental tool, potential for therapeutic use, and pursuit by the biotechnology industry. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current

Subjects

RNAi | RNA interface | therapeutics | siRNA | miRNA | shRNA

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.16 Experimental Molecular Biology: Biotechnology II (MIT)

Description

The course applies molecular biology and reverse genetics approaches to the study of apoptosis, or programmed cell death (PCD), in Drosophila cells. RNA interference (RNAi), or double stranded RNA-mediated gene silencing, will be used to inhibit expression of candidate apoptosis-related genes in cultured Drosophila cells. Teams of 2 or 3 students will design and carry out experiments to address questions about the genes involved in the regulation and execution of PCD in this system. Some projects involve the use of DNA damaging agents or other cytotoxic chemicals or drugs to help understand the pathways that control a cell's decision to undergo apoptosis. Instruction and practice in written and oral communication are provided.

Subjects

RNAi | RNA interference | programmed cell death | Drosophilia | PCD | mRNA | lab notebook | scientific writing | RT-PCR | S2 RNA | S2 | cell culture | genetic transcription | dsRNA | bioinformatics

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.344 RNA Interference: A New Tool for Genetic Analysis and Therapeutics (MIT)

Description

This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. To understand and treat any disease with a genetic basis or predisposition, scientists and clinicians need effective ways of manipulating the levels of genes and gene products. Conventional methods for the genetic modification of many experimental organisms are technically demanding and time consuming. Just over 5 years ago, a new mechanism of gene-silencing, termed RNA interference (RNAi), was discovered. In addition to being a fascinating biological process, RNAi provides a revolutionary technology that has a

Subjects

RNA interference | RNAi | RNA | genetic analysis | gene therapy | gene products | gene silencing | gene expression | human disease models | mRNA | genetic interference | short interfering RNA | siRNAs | expression vectors | RNA sequences | nucleotide fragments | microRNA | mRNA degradation | transgenic mice | lentivirus | knock-down animals | tissue specificity

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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HST.161 Molecular Biology and Genetics in Modern Medicine (MIT)

Description

This course provides a foundation for understanding the relationship between molecular biology, developmental biology, genetics, genomics, bioinformatics, and medicine. It develops explicit connections between basic research, medical understanding, and the perspective of patients. Principles of human genetics are reviewed. We translate clinical understanding into analysis at the level of the gene, chromosome and molecule; we cover the concepts and techniques of molecular biology and genomics, and the strategies and methods of genetic analysis, including an introduction to bioinformatics. Material in the course extends beyond basic principles to current research activity in human genetics.

Subjects

Genetics | genes | genetic disorders | inborn error | muscular dystrophy | PKU | phenylketoneuria | cancer | tumors | gene therapy | disease | birth defects | chromosomes | leukemia | RNAi | hemophilia | thalassemia | deafness | mutations | hypertrophic cardiomyopathy | epigenetics | rett syndrome | prenatal diagnosis | LOD scores | gene linkage | mitochondrial disorders | degenerative disorders | complex traits | Mendelian inheritance

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.342 The RNA Revolution: At the Frontiers of Cell Biology and Molecular Medicine (MIT)

Description

In this course, we will investigate the diverse types and functions of different RNA species, with a focus on "non-coding RNAs," i.e. those that do not directly encode proteins. The course will convey both the exciting discoveries in and frontiers of RNA research that are propelling our understanding of cell biology as well as the intellectual and experimental approaches responsible.The molecular biology revolution firmly established the role of DNA as the primary carrier of genetic information and proteins as the primary effector molecules of the cell. The intermediate between DNA and proteins is RNA, which initially was regarded as the "molecule in the middle" of the central dogma. This view has been transformed over the past two decades, as RNA has become recogn

Subjects

RNA | non-coding RNAs | ribosomal RNA | catalytic | circular RNA | long non-coding RNA | RNAi | RNA therapeutics | microRNAs | CRISPR/Cas9 | miRNAs | siRNA

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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