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7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT) 7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT)

Description

One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a

Subjects

Green Fluorescent Protein | Green Fluorescent Protein | Fluorescent protein engineering | Fluorescent protein engineering | Photoconversion | Photoconversion | fluorescent protein variants | fluorescent protein variants | fluorescent microscopy facility | fluorescent microscopy facility | Quantitative fluorescent imaging | Quantitative fluorescent imaging | ultra-sensitive fluorescent imaging | ultra-sensitive fluorescent imaging | high-throughput analysis | high-throughput analysis | Fluorescent imaging in living organisms | Fluorescent imaging in living organisms | phycoerythrin | phycoerythrin | phytochrome | phytochrome | jellyfish | jellyfish | red fluorescent protein | red fluorescent protein | photoactivation | photoactivation | chromophore | chromophore | protonation | protonation | lysosomes | lysosomes | recombinant protein molecules | recombinant protein molecules

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7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT)

Description

One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a

Subjects

Green Fluorescent Protein | Fluorescent protein engineering | Photoconversion | fluorescent protein variants | fluorescent microscopy facility | Quantitative fluorescent imaging | ultra-sensitive fluorescent imaging | high-throughput analysis | Fluorescent imaging in living organisms | phycoerythrin | phytochrome | jellyfish | red fluorescent protein | photoactivation | chromophore | protonation | lysosomes | recombinant protein molecules

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT)

Description

One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a

Subjects

Green Fluorescent Protein | Fluorescent protein engineering | Photoconversion | fluorescent protein variants | fluorescent microscopy facility | Quantitative fluorescent imaging | ultra-sensitive fluorescent imaging | high-throughput analysis | Fluorescent imaging in living organisms | phycoerythrin | phytochrome | jellyfish | red fluorescent protein | photoactivation | chromophore | protonation | lysosomes | recombinant protein molecules

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-allsimplifiedchinesecourses.xml

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