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Chemical analysis in the electron microscope

Description

This is a third edition of the Electron Microscopy and Analysis textbook, which was published by Taylor and Francis Books UK in 2001 (ISBN 0748409688). It deals with several sophisticated techniques for magnifying images of very small objects by large amounts - especially in a physical science context. Consisting of seven chapters, presented as separate files the resource incorporates questions and answers in each chapter for ease of learning. Equally as relevant for material scientists and bioscientists, this resource is an essential textbook and laboratory manual. The chapter describes principles of the chemical analysis in the SEM and TEM.

Subjects

electron microscopy | book | analysis | characteristic x-rays | x-ray spectrometry | microprobe analysis | thin film analysis | correction methods | eels | electron energy loss spectrometry | corematerials | ukoer | Engineering | H000

License

Attribution-Share Alike 2.0 UK: England & Wales Attribution-Share Alike 2.0 UK: England & Wales http://creativecommons.org/licenses/by-sa/2.0/uk/ http://creativecommons.org/licenses/by-sa/2.0/uk/

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12.141 Electron Microprobe Analysis (MIT) 12.141 Electron Microprobe Analysis (MIT)

Description

Introduction to the theory of x-ray microanalysis through the electron microprobe including ZAF matrix corrections. Techniques to be discussed are wavelength and energy dispersive spectrometry, scanning backscattered electron, secondary electron, cathodoluminescence, and X-ray imaging. Lab sessions involve hands-on use of the electron microprobe.Offered for undergraduate credit, but persons interested in an in-depth discussion of quantitative x-ray analysis are invited to participate. Students will be required to complete lab exercises to obtain credit. Introduction to the theory of x-ray microanalysis through the electron microprobe including ZAF matrix corrections. Techniques to be discussed are wavelength and energy dispersive spectrometry, scanning backscattered electron, secondary electron, cathodoluminescence, and X-ray imaging. Lab sessions involve hands-on use of the electron microprobe.Offered for undergraduate credit, but persons interested in an in-depth discussion of quantitative x-ray analysis are invited to participate. Students will be required to complete lab exercises to obtain credit.

Subjects

x-ray microanalysis | x-ray microanalysis | electron microprobe | electron microprobe | ZAF matrix corrections | ZAF matrix corrections | wavelength and energy dispersive spectrometry | wavelength and energy dispersive spectrometry | scanning backscattered electron | scanning backscattered electron | secondary electron | secondary electron | cathodoluminescence | cathodoluminescence | X-ray imaging | X-ray imaging

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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5.13 Organic Chemistry II (MIT) 5.13 Organic Chemistry II (MIT)

Description

This intermediate organic chemistry course focuses on the methods used to identify the structure of organic molecules, advanced principles of organic stereochemistry, organic reaction mechanisms, and methods used for the synthesis of organic compounds. Additional special topics include illustrating the role of organic chemistry in biology, medicine, and industry. This intermediate organic chemistry course focuses on the methods used to identify the structure of organic molecules, advanced principles of organic stereochemistry, organic reaction mechanisms, and methods used for the synthesis of organic compounds. Additional special topics include illustrating the role of organic chemistry in biology, medicine, and industry.

Subjects

intermediate organic chemistry | intermediate organic chemistry | organic molecules | organic molecules | stereochemistry | stereochemistry | reaction mechanisms | reaction mechanisms | synthesis of organic compounds | synthesis of organic compounds | synthesis | synthesis | structure determination | structure determination | mechanism | mechanism | reactivity | reactivity | functional groups | functional groups | NMR | NMR | spectroscopy | spectroscopy | spectrometry | spectrometry | structure elucidation | structure elucidation | infrared spectroscopy | infrared spectroscopy | nuclear magnetic resonance spectroscopy | nuclear magnetic resonance spectroscopy | reactive intermediates | reactive intermediates | carbocations | carbocations | radicals | radicals | aromaticity | aromaticity | conjugated systems | conjugated systems | molecular orbital theory | molecular orbital theory | pericyclic reactions | pericyclic reactions

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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5.32 Intermediate Chemical Experimentation (MIT) 5.32 Intermediate Chemical Experimentation (MIT)

Description

5.32 involves more advanced experimental work than 5.310 or 5.311. The course emphasizes organic synthesis assisted by chiral catalysis, purification, and analysis of organic compounds employing such methods as IR, 1D and 2D NMR, UV spectroscopies and mass spectrometry, and thin layer and non-chiral and chiral gas chromatography. In 5.32, experiments also involve enzyme purification, characterization and assays, as well as molecular modeling in organic synthesis and in biochemical systems. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such 5.32 involves more advanced experimental work than 5.310 or 5.311. The course emphasizes organic synthesis assisted by chiral catalysis, purification, and analysis of organic compounds employing such methods as IR, 1D and 2D NMR, UV spectroscopies and mass spectrometry, and thin layer and non-chiral and chiral gas chromatography. In 5.32, experiments also involve enzyme purification, characterization and assays, as well as molecular modeling in organic synthesis and in biochemical systems. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such

Subjects

intermediate chemical experimentation | intermediate chemical experimentation | experiment | experiment | chemistry | chemistry | organic synthesis | organic synthesis | chiral catalysis | chiral catalysis | purification | purification | organic chemistry | organic chemistry | laboratory | laboratory | IR | IR | 1D NMR | 1D NMR | 2D NMR | 2D NMR | UV spectroscopy | UV spectroscopy | mass spectrometry | mass spectrometry | thin layer gas chromatography | thin layer gas chromatography | non-chiral gas chromatography | non-chiral gas chromatography | chiral gas chromatography | chiral gas chromatography | enzyme purification | enzyme purification | characterization | characterization | assays | assays | molecular modeling | molecular modeling | biochemical systems | biochemical systems

License

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12.141 Electron Microprobe Analysis by Wavelength Dispersive X-ray Spectrometry (MIT) 12.141 Electron Microprobe Analysis by Wavelength Dispersive X-ray Spectrometry (MIT)

Description

This lab-oriented course introduces the student to the subject of X-ray spectrometry and micro-scale chemical quantitative analysis of solid samples through an intensive series of hands-on laboratory exercises that use the electron microprobe. This lab-oriented course introduces the student to the subject of X-ray spectrometry and micro-scale chemical quantitative analysis of solid samples through an intensive series of hands-on laboratory exercises that use the electron microprobe.

Subjects

x-ray microanalysis | x-ray microanalysis | electron microprobe | electron microprobe | ZAF matrix corrections | ZAF matrix corrections | wavelength and energy dispersive spectrometry | wavelength and energy dispersive spectrometry | scanning backscattered electron | scanning backscattered electron | secondary electron | secondary electron | cathodoluminescence | cathodoluminescence | and X-ray imaging | and X-ray imaging

License

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12.158 Molecular Biogeochemistry (MIT) 12.158 Molecular Biogeochemistry (MIT)

Description

This course covers all aspects of molecular biosignatures, such as their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record. Students analyze in depth the recent literature on chemical fossils. Lectures provide background on the subject matter. Basic knowledge of organic chemistry required. Students taking graduate version complete additional assignments. This course covers all aspects of molecular biosignatures, such as their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record. Students analyze in depth the recent literature on chemical fossils. Lectures provide background on the subject matter. Basic knowledge of organic chemistry required. Students taking graduate version complete additional assignments.

Subjects

molecular biogeochemistry | molecular biogeochemistry | biosynthesis | biosynthesis | phylogenetic origins | phylogenetic origins | acetogenic lipids | acetogenic lipids | acylic isoprenoids | acylic isoprenoids | molecular biosugnatures | molecular biosugnatures | steroids | steroids | mass spectrometry | mass spectrometry

License

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20.320 Analysis of Biomolecular and Cellular Systems (MIT) 20.320 Analysis of Biomolecular and Cellular Systems (MIT)

Description

This course focuses on computational and experimental analysis of biological systems across a hierarchy of scales, including genetic, molecular, cellular, and cell population levels. The two central themes of the course are modeling of complex dynamic systems and protein design and engineering. Topics include gene sequence analysis, molecular modeling, metabolic and gene regulation networks, signal transduction pathways and cell populations in tissues. Emphasis is placed on experimental methods, quantitative analysis, and computational modeling. This course focuses on computational and experimental analysis of biological systems across a hierarchy of scales, including genetic, molecular, cellular, and cell population levels. The two central themes of the course are modeling of complex dynamic systems and protein design and engineering. Topics include gene sequence analysis, molecular modeling, metabolic and gene regulation networks, signal transduction pathways and cell populations in tissues. Emphasis is placed on experimental methods, quantitative analysis, and computational modeling.

Subjects

biological engineering | biological engineering | kinase | kinase | PyMOL | PyMOL | PyRosetta | PyRosetta | MATLAB | MATLAB | Michaelis-Menten | Michaelis-Menten | bioreactor | bioreactor | bromodomain | bromodomain | protein-ligand interactions | protein-ligand interactions | titration analysis | titration analysis | fractional separation | fractional separation | isothermal titration calorimetry | isothermal titration calorimetry | ITC | ITC | mass spectrometry | mass spectrometry | MS | MS | co-immunoprecipitation | co-immunoprecipitation | Co-IP | Co-IP | Forster resonance energy transfer | Forster resonance energy transfer | FRET | FRET | primary ligation assay | primary ligation assay | PLA | PLA | surface plasmon resonance | surface plasmon resonance | SPR | SPR | enzyme kinetics | enzyme kinetics | kinase engineering | kinase engineering | competitive inhibition | competitive inhibition | epidermal growth factor receptor | epidermal growth factor receptor | mitogen-activated protein kinase | mitogen-activated protein kinase | MAPK | MAPK | genome editing | genome editing | Imatinib | Imatinib | Gleevec | Gleevec | Glivec | Glivec | drug delivery | drug delivery | kinetics of molecular processes | kinetics of molecular processes | dynamics of molecular processes | dynamics of molecular processes | kinetics of cellular processes | kinetics of cellular processes | dynamics of cellular processes | dynamics of cellular processes | intracellular scale | intracellular scale | extracellular scale | extracellular scale | and cell population scale | and cell population scale | biotechnology applications | biotechnology applications | gene regulation networks | gene regulation networks | nucleic acid hybridization | nucleic acid hybridization | signal transduction pathways | signal transduction pathways | cell populations in tissues | cell populations in tissues | cell populations in bioreactors | cell populations in bioreactors | experimental methods | experimental methods | quantitative analysis | quantitative analysis | computational modeling | computational modeling

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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12.744 Marine Isotope Chemistry (MIT) 12.744 Marine Isotope Chemistry (MIT)

Description

The objective of this course is to develop an understanding of principles of marine isotope geochemistry, its systematics, and its application to the study of the behavior and history of the oceans within the earth system. The emphasis is on developing the underlying concepts and theory as well as proficiency in working with practical isotope systems. The course is divided into four sections: nuclear systematics, Earth formation and evolution, stable isotopes, and applications to the ocean system. The objective of this course is to develop an understanding of principles of marine isotope geochemistry, its systematics, and its application to the study of the behavior and history of the oceans within the earth system. The emphasis is on developing the underlying concepts and theory as well as proficiency in working with practical isotope systems. The course is divided into four sections: nuclear systematics, Earth formation and evolution, stable isotopes, and applications to the ocean system.

Subjects

oceanography | oceanography | chemical oceanography | chemical oceanography | isotope geochemistry | isotope geochemistry | geochemistry | geochemistry | marine science | marine science | isotopes | isotopes | radiocarbon | radiocarbon | radioactive decay | radioactive decay | radiometric dating | radiometric dating | mass spectrometry | mass spectrometry | isotope fractionation | isotope fractionation | fractionation | fractionation | water column | water column | whoi | whoi | woods hole | woods hole | earth science | earth science | atmosphere | atmosphere | ocean | ocean

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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12.158 Molecular Biogeochemistry (MIT) 12.158 Molecular Biogeochemistry (MIT)

Description

This course covers all aspects of molecular biosignatures, such as their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record. Students analyze in depth the recent literature on chemical fossils. Lectures provide background on the subject matter. Basic knowledge of organic chemistry required. Students taking graduate version complete additional assignments. This course covers all aspects of molecular biosignatures, such as their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record. Students analyze in depth the recent literature on chemical fossils. Lectures provide background on the subject matter. Basic knowledge of organic chemistry required. Students taking graduate version complete additional assignments.

Subjects

molecular biogeochemistry | molecular biogeochemistry | biosynthesis | biosynthesis | phylogenetic origins | phylogenetic origins | acetogenic lipids | acetogenic lipids | acylic isoprenoids | acylic isoprenoids | molecular biosugnatures | molecular biosugnatures | steroids | steroids | mass spectrometry | mass spectrometry

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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12.141 Electron Microprobe Analysis (MIT) 12.141 Electron Microprobe Analysis (MIT)

Description

The electron microprobe provides a complete micrometer-scale quantitative chemical analysis of inorganic solids. The method is nondestructive and utilizes characteristic X-rays excited by an electron beam incident on a flat surface of the sample. This course provides an introduction to the theory of X-ray microanalysis through wavelength and energy dispersive spectrometry (WDS and EDS), ZAF matrix correction procedures and scanning electron imaging with back-scattered electron (BSE), secondary electron (SE), X-ray using WDS or EDS (elemental mapping), and cathodoluminescence (CL). Lab sessions involve hands-on use of the JEOL JXA-8200 Superprobe. The electron microprobe provides a complete micrometer-scale quantitative chemical analysis of inorganic solids. The method is nondestructive and utilizes characteristic X-rays excited by an electron beam incident on a flat surface of the sample. This course provides an introduction to the theory of X-ray microanalysis through wavelength and energy dispersive spectrometry (WDS and EDS), ZAF matrix correction procedures and scanning electron imaging with back-scattered electron (BSE), secondary electron (SE), X-ray using WDS or EDS (elemental mapping), and cathodoluminescence (CL). Lab sessions involve hands-on use of the JEOL JXA-8200 Superprobe.

Subjects

electron microprobe | electron microprobe | x-ray microanalysis | x-ray microanalysis | x-ray imaging | x-ray imaging | ZAF matrix corrections | ZAF matrix corrections | wavelength | wavelength | energy dispersive spectrometry | energy dispersive spectrometry | scanning backscattered electron | scanning backscattered electron | secondary electron | secondary electron | cathodoluminescence | cathodoluminescence

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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12.458 Molecular Biogeochemistry (MIT) 12.458 Molecular Biogeochemistry (MIT)

Description

This course evaluates and discusses the formation and diagnostic structural properties of organic compounds with particular emphasis on those molecules which form chemical fossils. The course is structured around the biosynthetic and phylogenetic origins of recalcitrant hydrocarbons. This course evaluates and discusses the formation and diagnostic structural properties of organic compounds with particular emphasis on those molecules which form chemical fossils. The course is structured around the biosynthetic and phylogenetic origins of recalcitrant hydrocarbons.

Subjects

molecular biogeochemistry | molecular biogeochemistry | biosynthesis | biosynthesis | phylogenetic origins | phylogenetic origins | acetogenic lipids | acetogenic lipids | acylic isoprenoids | acylic isoprenoids | molecular biosugnatures | molecular biosugnatures | steroids | steroids | mass spectrometry | mass spectrometry

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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12.158 Molecular Biogeochemistry (MIT)

Description

This course covers all aspects of molecular biosignatures, such as their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record. Students analyze in depth the recent literature on chemical fossils. Lectures provide background on the subject matter. Basic knowledge of organic chemistry required. Students taking graduate version complete additional assignments.

Subjects

molecular biogeochemistry | biosynthesis | phylogenetic origins | acetogenic lipids | acylic isoprenoids | molecular biosugnatures | steroids | mass spectrometry

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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Laboratory Investigation of Chewing Gum

Description

A laboratory-based investigation into the chemical flavourings found in chewing gum. The investigation is based around some 'problem' gum and the students are set the challenge of solving the problem. Students contribute to experimental design and carry out extractions, purifications and analysis of purified extracts by GC and/or GC-MS.

Subjects

chewing gum | chemistry | flavourings | problem solving | laboratory | extractions | gas chromatography | mass spectrometry | ukoer | sfsoer | Physical sciences | F000

License

Attribution-Noncommercial-Share Alike 2.0 UK: England & Wales Attribution-Noncommercial-Share Alike 2.0 UK: England & Wales http://creativecommons.org/licenses/by-nc-sa/2.0/uk/ http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

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Laboratory Investigation of Fatty Acid Supplements

Description

This is a laboratory-based investigation of so-called essential fatty acids (e.g. omega-3,6 fatty acids) in supplements. Students carry out extractions and analyse extracts by GC and/or GC/MS. They also carry out small scale derivatisation reactions and purifications. Data is analaysed wuth respect to composition of individual supplements, comparison with packaging information, etc.

Subjects

chemistry | laboratory | fatty acids | food | analytical | gas chromatography | mass spectrometry | sfsoer | ukoer | Physical sciences | F000

License

Attribution-Noncommercial-Share Alike 2.0 UK: England & Wales Attribution-Noncommercial-Share Alike 2.0 UK: England & Wales http://creativecommons.org/licenses/by-nc-sa/2.0/uk/ http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

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5.13 Organic Chemistry II (MIT)

Description

This intermediate organic chemistry course focuses on the methods used to identify the structure of organic molecules, advanced principles of organic stereochemistry, organic reaction mechanisms, and methods used for the synthesis of organic compounds. Additional special topics include illustrating the role of organic chemistry in biology, medicine, and industry.

Subjects

intermediate organic chemistry | organic molecules | stereochemistry | reaction mechanisms | synthesis of organic compounds | synthesis | structure determination | mechanism | reactivity | functional groups | NMR | spectroscopy | spectrometry | structure elucidation | infrared spectroscopy | nuclear magnetic resonance spectroscopy | reactive intermediates | carbocations | radicals | aromaticity | conjugated systems | molecular orbital theory | pericyclic reactions

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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12.458 Molecular Biogeochemistry (MIT)

Description

This course evaluates and discusses the formation and diagnostic structural properties of organic compounds with particular emphasis on those molecules which form chemical fossils. The course is structured around the biosynthetic and phylogenetic origins of recalcitrant hydrocarbons.

Subjects

molecular biogeochemistry | biosynthesis | phylogenetic origins | acetogenic lipids | acylic isoprenoids | molecular biosugnatures | steroids | mass spectrometry

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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5.13 Organic Chemistry II (MIT)

Description

This intermediate organic chemistry course focuses on the methods used to identify the structure of organic molecules, advanced principles of organic stereochemistry, organic reaction mechanisms, and methods used for the synthesis of organic compounds. Additional special topics include illustrating the role of organic chemistry in biology, medicine, and industry.

Subjects

intermediate organic chemistry | organic molecules | stereochemistry | reaction mechanisms | synthesis of organic compounds | synthesis | structure determination | mechanism | reactivity | functional groups | NMR | spectroscopy | spectrometry | structure elucidation | infrared spectroscopy | nuclear magnetic resonance spectroscopy | reactive intermediates | carbocations | radicals | aromaticity | conjugated systems | molecular orbital theory | pericyclic reactions

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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12.744 Marine Isotope Chemistry (MIT)

Description

The objective of this course is to develop an understanding of principles of marine isotope geochemistry, its systematics, and its application to the study of the behavior and history of the oceans within the earth system. The emphasis is on developing the underlying concepts and theory as well as proficiency in working with practical isotope systems. The course is divided into four sections: nuclear systematics, Earth formation and evolution, stable isotopes, and applications to the ocean system.

Subjects

oceanography | chemical oceanography | isotope geochemistry | geochemistry | marine science | isotopes | radiocarbon | radioactive decay | radiometric dating | mass spectrometry | isotope fractionation | fractionation | water column | whoi | woods hole | earth science | atmosphere | ocean

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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Introduction to Molecular Spectroscopy

Description

This guide provides an introduction to the different types of molecular spectroscopic analysis, describing the basic principles of each technique and practical considerations including sample preparation. It is illustrated with simple diagrams, photographs of equipment and information to aid interpretation of spectra.

Subjects

ukoer | sfsoer | spectroscopy | molecular | absorption | infrared | fluorescence | mass spectrometry | analytical | uv-vis | ftir | ft-ir | ms | nmr | Physical sciences | F000

License

Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales http://creativecommons.org/licenses/by-nc-nd/2.0/uk/ http://creativecommons.org/licenses/by-nc-nd/2.0/uk/

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Introduction to Atomic Spectrometry

Description

An introduction to atomic spectrometric analysis, describing the basic principles of each technique, their application areas and modes of operation, including practical comparison of the techniques. It is illustrated with simple diagrams and photographs of equipment.

Subjects

ukoer | sfsoer | atomic spectrometry | icp-aes | icp-oes | icp-ms | xrf | aas | Physical sciences | F000

License

Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales http://creativecommons.org/licenses/by-nc-nd/2.0/uk/ http://creativecommons.org/licenses/by-nc-nd/2.0/uk/

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5.32 Intermediate Chemical Experimentation (MIT)

Description

5.32 involves more advanced experimental work than 5.310 or 5.311. The course emphasizes organic synthesis assisted by chiral catalysis, purification, and analysis of organic compounds employing such methods as IR, 1D and 2D NMR, UV spectroscopies and mass spectrometry, and thin layer and non-chiral and chiral gas chromatography. In 5.32, experiments also involve enzyme purification, characterization and assays, as well as molecular modeling in organic synthesis and in biochemical systems. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such

Subjects

intermediate chemical experimentation | experiment | chemistry | organic synthesis | chiral catalysis | purification | organic chemistry | laboratory | IR | 1D NMR | 2D NMR | UV spectroscopy | mass spectrometry | thin layer gas chromatography | non-chiral gas chromatography | chiral gas chromatography | enzyme purification | characterization | assays | molecular modeling | biochemical systems

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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Analytical Chemistry Techniques Manual

Description

A set of four separate guides which provide introductions to the principles and practical application of different analytical chemistry techniques, illustrated with simple diagrams and photographs of equipment: (1) introduction to analytical chemistry, focusing on the analytical process, quality assurance and general laboratory techniques e.g. solution preparation; (2) introduction to molecular spectroscopy, including UV-Vis, fluorescence, IR, MS and NMR spectroscopy; (3) introduction to chromatography, including TLC, GC, HPLC and ion chromatography; (4) introduction to atomic spectrometry, including ICP-AES, ICP-MS, XRF and AAS. Each guide is also available for separate download as a pdf file.

Subjects

ukoer | sfsoer | analytical chemistry | analytical science | chromatography | spectroscopy | spectrometry | Physical sciences | F000

License

Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales http://creativecommons.org/licenses/by-nc-nd/2.0/uk/ http://creativecommons.org/licenses/by-nc-nd/2.0/uk/

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20.320 Analysis of Biomolecular and Cellular Systems (MIT)

Description

This course focuses on computational and experimental analysis of biological systems across a hierarchy of scales, including genetic, molecular, cellular, and cell population levels. The two central themes of the course are modeling of complex dynamic systems and protein design and engineering. Topics include gene sequence analysis, molecular modeling, metabolic and gene regulation networks, signal transduction pathways and cell populations in tissues. Emphasis is placed on experimental methods, quantitative analysis, and computational modeling.

Subjects

biological engineering | kinase | PyMOL | PyRosetta | MATLAB | Michaelis-Menten | bioreactor | bromodomain | protein-ligand interactions | titration analysis | fractional separation | isothermal titration calorimetry | ITC | mass spectrometry | MS | co-immunoprecipitation | Co-IP | Forster resonance energy transfer | FRET | primary ligation assay | PLA | surface plasmon resonance | SPR | enzyme kinetics | kinase engineering | competitive inhibition | epidermal growth factor receptor | mitogen-activated protein kinase | MAPK | genome editing | Imatinib | Gleevec | Glivec | drug delivery | kinetics of molecular processes | dynamics of molecular processes | kinetics of cellular processes | dynamics of cellular processes | intracellular scale | extracellular scale | and cell population scale | biotechnology applications | gene regulation networks | nucleic acid hybridization | signal transduction pathways | cell populations in tissues | cell populations in bioreactors | experimental methods | quantitative analysis | computational modeling

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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5.32 Intermediate Chemical Experimentation (MIT)

Description

5.32 involves more advanced experimental work than 5.310 or 5.311. The course emphasizes organic synthesis assisted by chiral catalysis, purification, and analysis of organic compounds employing such methods as IR, 1D and 2D NMR, UV spectroscopies and mass spectrometry, and thin layer and non-chiral and chiral gas chromatography. In 5.32, experiments also involve enzyme purification, characterization and assays, as well as molecular modeling in organic synthesis and in biochemical systems. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such

Subjects

intermediate chemical experimentation | experiment | chemistry | organic synthesis | chiral catalysis | purification | organic chemistry | laboratory | IR | 1D NMR | 2D NMR | UV spectroscopy | mass spectrometry | thin layer gas chromatography | non-chiral gas chromatography | chiral gas chromatography | enzyme purification | characterization | assays | molecular modeling | biochemical systems

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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12.158 Molecular Biogeochemistry (MIT)

Description

This course covers all aspects of molecular biosignatures, such as their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record. Students analyze in depth the recent literature on chemical fossils. Lectures provide background on the subject matter. Basic knowledge of organic chemistry required. Students taking graduate version complete additional assignments.

Subjects

molecular biogeochemistry | biosynthesis | phylogenetic origins | acetogenic lipids | acylic isoprenoids | molecular biosugnatures | steroids | mass spectrometry

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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