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Communication (MIT) Communication (MIT)

Description

This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop

Subjects

experimental biology | experimental biology | microbial genetics | microbial genetics | protein biochemistry | protein biochemistry | recombinant DNA | recombinant DNA | development | development | zebrafish | zebrafish | phase contrast microscopy | phase contrast microscopy | teratogenesis | teratogenesis | rna isolation | rna isolation | northern blot | northern blot | gene expression | gene expression | western blot | western blot | PCR | PCR | polymerase chain reaction | polymerase chain reaction | RNA gel | RNA gel | RNA fixation | RNA fixation | probe labeling | probe labeling | mutagenesis | mutagenesis | transposon | transposon | column chromatography | column chromatography | size-exclusion chromatography | size-exclusion chromatography | anion exchange chromatography | anion exchange chromatography | SDS-Page gel | SDS-Page gel | enzyme kinetics | enzyme kinetics | transformation | transformation | primers | primers

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.13 Experimental Microbial Genetics (MIT) 7.13 Experimental Microbial Genetics (MIT)

Description

In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production. Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized. WARNING NOTICE The experiments described in these materials In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production. Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized. WARNING NOTICE The experiments described in these materials

Subjects

microbiology | microbiology | genetics | genetics | pseudomonas | pseudomonas | bacteria | bacteria | genes | genes | pathogen | pathogen | mutagenesis | mutagenesis | PCR | PCR | DNA sequencing | DNA sequencing | enzyme assays | enzyme assays | gene expression | gene expression | molecular genetics | molecular genetics | plasmid manipulation | plasmid manipulation | genetic complementation | genetic complementation | laboratory | laboratory | protocol | protocol | vector | vector | mutant | mutant | cystic fibrosis | cystic fibrosis

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.344 Directed Evolution: Engineering Biocatalysts (MIT) 7.344 Directed Evolution: Engineering Biocatalysts (MIT)

Description

Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current

Subjects

evolution | evolution | biocatalyst | biocatalyst | mutation | mutation | library | library | recombination | recombination | directed evolution | directed evolution | enzyme | enzyme | point mutation | point mutation | mutagenesis | mutagenesis | DNA | DNA | gene | gene | complementation | complementation | affinity | affinity | phage | phage | ribosome display | ribosome display | yeast surface display | yeast surface display | bacterial cell surface display | bacterial cell surface display | IVC | IVC | FACS | FACS | active site | active site

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.13 Experimental Microbial Genetics (MIT) 7.13 Experimental Microbial Genetics (MIT)

Description

Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology. Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology.

Subjects

microbiology | microbiology | genetics | genetics | rhodococcus | rhodococcus | bacteria | bacteria | genes | genes | plasmid manipulation | plasmid manipulation | mutagenesis | mutagenesis | PCR | PCR | DNA sequencing | DNA sequencing | enzyme assays | enzyme assays | gene expression | gene expression | molecular genetics | molecular genetics | Gram-positive | Gram-positive | gram-negative | gram-negative | bioconversion processes | bioconversion processes | synthesis | synthesis | precursors | precursors | metabolites | metabolites | genetic complementation | genetic complementation | laboratory | laboratory | lab | lab

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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10.492-2 Integrated Chemical Engineering Topics I: Introduction to Biocatalysis (MIT) 10.492-2 Integrated Chemical Engineering Topics I: Introduction to Biocatalysis (MIT)

Description

This course provides a brief introduction to the field of biocatalysis in the context of process design. Fundamental topics include why and when one may choose to use biological systems for chemical conversion, considerations for using free enzymes versus whole cells, and issues related to design and development of bioconversion processes. Biological and engineering problems are discussed as well as how one may arrive at both biological and engineering solutions. This course provides a brief introduction to the field of biocatalysis in the context of process design. Fundamental topics include why and when one may choose to use biological systems for chemical conversion, considerations for using free enzymes versus whole cells, and issues related to design and development of bioconversion processes. Biological and engineering problems are discussed as well as how one may arrive at both biological and engineering solutions.

Subjects

biocatalysis | biocatalysis | enzymes | enzymes | enzyme kinetics | enzyme kinetics | whole cell catalysts | whole cell catalysts | biocatalytic processes | biocatalytic processes | site-directed mutagenesis | site-directed mutagenesis | cloning | cloning | enzyme performance | enzyme performance | enzyme specificity | enzyme specificity | enzyme inhibition | enzyme inhibition | enzyme toxicity | enzyme toxicity | yield | yield | enzyme instability | enzyme instability | equilibrium reactions | equilibrium reactions | product solubility | product solubility | substrate solubility | substrate solubility

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.13 Experimental Microbial Genetics (MIT) 7.13 Experimental Microbial Genetics (MIT)

Description

Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology. Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology.

Subjects

microbiology | microbiology | genetics | genetics | rhodococcus | rhodococcus | bacteria | bacteria | genes | genes | plasmid manipulation | plasmid manipulation | mutagenesis | mutagenesis | PCR | PCR | DNA sequencing | DNA sequencing | enzyme assays | enzyme assays | gene expression | gene expression | molecular genetics | molecular genetics | Gram-positive | Gram-positive | gram-negative | gram-negative | bioconversion processes | bioconversion processes | synthesis | synthesis | precursors | precursors | metabolites | metabolites | genetic complementation | genetic complementation | laboratory | laboratory | lab | lab

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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Communication (MIT)

Description

This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop

Subjects

experimental biology | microbial genetics | protein biochemistry | recombinant DNA | development | zebrafish | phase contrast microscopy | teratogenesis | rna isolation | northern blot | gene expression | western blot | PCR | polymerase chain reaction | RNA gel | RNA fixation | probe labeling | mutagenesis | transposon | column chromatography | size-exclusion chromatography | anion exchange chromatography | SDS-Page gel | enzyme kinetics | transformation | primers

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-allsimplifiedchinesecourses.xml

Attribution

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10.492-2 Integrated Chemical Engineering Topics I: Introduction to Biocatalysis (MIT)

Description

This course provides a brief introduction to the field of biocatalysis in the context of process design. Fundamental topics include why and when one may choose to use biological systems for chemical conversion, considerations for using free enzymes versus whole cells, and issues related to design and development of bioconversion processes. Biological and engineering problems are discussed as well as how one may arrive at both biological and engineering solutions.

Subjects

biocatalysis | enzymes | enzyme kinetics | whole cell catalysts | biocatalytic processes | site-directed mutagenesis | cloning | enzyme performance | enzyme specificity | enzyme inhibition | enzyme toxicity | yield | enzyme instability | equilibrium reactions | product solubility | substrate solubility

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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https://ocw.mit.edu/rss/all/mit-allsimplifiedchinesecourses.xml

Attribution

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7.13 Experimental Microbial Genetics (MIT)

Description

Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology.

Subjects

microbiology | genetics | rhodococcus | bacteria | genes | plasmid manipulation | mutagenesis | PCR | DNA sequencing | enzyme assays | gene expression | molecular genetics | Gram-positive | gram-negative | bioconversion processes | synthesis | precursors | metabolites | genetic complementation | laboratory | lab

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-allsimplifiedchinesecourses.xml

Attribution

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7.13 Experimental Microbial Genetics (MIT)

Description

In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production. Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized. WARNING NOTICE The experiments described in these materials

Subjects

microbiology | genetics | pseudomonas | bacteria | genes | pathogen | mutagenesis | PCR | DNA sequencing | enzyme assays | gene expression | molecular genetics | plasmid manipulation | genetic complementation | laboratory | protocol | vector | mutant | cystic fibrosis

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-alllifesciencescourses.xml

Attribution

Click to get HTML | Click to get attribution | Click to get URL

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7.344 Directed Evolution: Engineering Biocatalysts (MIT)

Description

Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current

Subjects

evolution | biocatalyst | mutation | library | recombination | directed evolution | enzyme | point mutation | mutagenesis | DNA | gene | complementation | affinity | phage | ribosome display | yeast surface display | bacterial cell surface display | IVC | FACS | active site

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-alllifesciencescourses.xml

Attribution

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Communication (MIT)

Description

This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop

Subjects

experimental biology | microbial genetics | protein biochemistry | recombinant DNA | development | zebrafish | phase contrast microscopy | teratogenesis | rna isolation | northern blot | gene expression | western blot | PCR | polymerase chain reaction | RNA gel | RNA fixation | probe labeling | mutagenesis | transposon | column chromatography | size-exclusion chromatography | anion exchange chromatography | SDS-Page gel | enzyme kinetics | transformation | primers

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-allcourses.xml

Attribution

Click to get HTML | Click to get attribution | Click to get URL

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7.13 Experimental Microbial Genetics (MIT)

Description

Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology.

Subjects

microbiology | genetics | rhodococcus | bacteria | genes | plasmid manipulation | mutagenesis | PCR | DNA sequencing | enzyme assays | gene expression | molecular genetics | Gram-positive | gram-negative | bioconversion processes | synthesis | precursors | metabolites | genetic complementation | laboratory | lab

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-allcourses.xml

Attribution

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10.492-2 Integrated Chemical Engineering Topics I: Introduction to Biocatalysis (MIT)

Description

This course provides a brief introduction to the field of biocatalysis in the context of process design. Fundamental topics include why and when one may choose to use biological systems for chemical conversion, considerations for using free enzymes versus whole cells, and issues related to design and development of bioconversion processes. Biological and engineering problems are discussed as well as how one may arrive at both biological and engineering solutions.

Subjects

biocatalysis | enzymes | enzyme kinetics | whole cell catalysts | biocatalytic processes | site-directed mutagenesis | cloning | enzyme performance | enzyme specificity | enzyme inhibition | enzyme toxicity | yield | enzyme instability | equilibrium reactions | product solubility | substrate solubility

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-allcourses.xml

Attribution

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7.13 Experimental Microbial Genetics (MIT)

Description

Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology.

Subjects

microbiology | genetics | rhodococcus | bacteria | genes | plasmid manipulation | mutagenesis | PCR | DNA sequencing | enzyme assays | gene expression | molecular genetics | Gram-positive | gram-negative | bioconversion processes | synthesis | precursors | metabolites | genetic complementation | laboratory | lab

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

Site sourced from

https://ocw.mit.edu/rss/all/mit-alltraditionalchinesecourses.xml

Attribution

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