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7.01SC Fundamentals of Biology (MIT) 7.01SC Fundamentals of Biology (MIT)

Description

Fundamentals of Biology focuses on the basic principles of biochemistry, molecular biology, genetics, and recombinant DNA. These principles are necessary to understanding the basic mechanisms of life and anchor the biological knowledge that is required to understand many of the challenges in everyday life, from human health and disease to loss of biodiversity and environmental quality. Fundamentals of Biology focuses on the basic principles of biochemistry, molecular biology, genetics, and recombinant DNA. These principles are necessary to understanding the basic mechanisms of life and anchor the biological knowledge that is required to understand many of the challenges in everyday life, from human health and disease to loss of biodiversity and environmental quality.

Subjects

amino acids | amino acids | carboxyl group | carboxyl group | amino group | amino group | side chains | side chains | polar | polar | hydrophobic | hydrophobic | primary structure | primary structure | secondary structure | secondary structure | tertiary structure | tertiary structure | quaternary structure | quaternary structure | x-ray crystallography | x-ray crystallography | alpha helix | alpha helix | beta sheet | beta sheet | ionic bond | ionic bond | non-polar bond | non-polar bond | van der Waals interactions | van der Waals interactions | proton gradient | proton gradient | cyclic photophosphorylation | cyclic photophosphorylation | sunlight | sunlight | ATP | ATP | chlorophyll | chlorophyll | chlorophyll a | chlorophyll a | electrons | electrons | hydrogen sulfide | hydrogen sulfide | biosynthesis | biosynthesis | non-cyclic photophosphorylation | non-cyclic photophosphorylation | photosystem II | photosystem II | photosystem I | photosystem I | cyanobacteria | cyanobacteria | chloroplast | chloroplast | stroma | stroma | thylakoid membrane | thylakoid membrane | Genetics | Genetics | Mendel | Mendel | Mendel's Laws | Mendel's Laws | cloning | cloning | restriction enzymes | restriction enzymes | vector | vector | insert DNA | insert DNA | ligase | ligase | library | library | E.Coli | E.Coli | phosphatase | phosphatase | yeast | yeast | transformation | transformation | ARG1 gene | ARG1 gene | ARG1 mutant yeast | ARG1 mutant yeast | yeast wild-type | yeast wild-type | cloning by complementation | cloning by complementation | Human Beta Globin gene | Human Beta Globin gene | protein tetramer | protein tetramer | vectors | vectors | antibodies | antibodies | human promoter | human promoter | splicing | splicing | mRNA | mRNA | cDNA | cDNA | reverse transcriptase | reverse transcriptase | plasmid | plasmid | electrophoresis | electrophoresis | DNA sequencing | DNA sequencing | primer | primer | template | template | capillary tube | capillary tube | laser detector | laser detector | human genome project | human genome project | recombinant DNA | recombinant DNA | clone | clone | primer walking | primer walking | subcloning | subcloning | computer assembly | computer assembly | shotgun sequencing | shotgun sequencing | open reading frame | open reading frame | databases | databases | polymerase chain reaction (PCR) | polymerase chain reaction (PCR) | polymerase | polymerase | nucleotides | nucleotides | Thermus aquaticus | Thermus aquaticus | Taq polymerase | Taq polymerase | thermocycler | thermocycler | resequencing | resequencing | in vitro fertilization | in vitro fertilization | pre-implantation diagnostics | pre-implantation diagnostics | forensics | forensics | genetic engineering | genetic engineering | DNA sequences | DNA sequences | therapeutic proteins | therapeutic proteins | E. coli | E. coli | disease-causing mutations | disease-causing mutations | cleavage of DNA | cleavage of DNA | bacterial transformation | bacterial transformation | recombinant DNA revolution | recombinant DNA revolution | biotechnology industry | biotechnology industry | Robert Swanson | Robert Swanson | toxin gene | toxin gene | pathogenic bacterium | pathogenic bacterium | biomedical research | biomedical research | S. Pyogenes | S. Pyogenes | origin of replication | origin of replication

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.013 Introductory Biology (MIT) 7.013 Introductory Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.013 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer), The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.013 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer),

Subjects

biology | biology | biochemistry | biochemistry | genetics | genetics | molecular biology | molecular biology | recombinant DNA | recombinant DNA | cell cycle | cell cycle | cell signaling | cell signaling | cloning | cloning | stem cells | stem cells | cancer | cancer | immunology | immunology | virology | virology | genomics | genomics | molecular medicine | molecular medicine | DNA | DNA | RNA | RNA | proteins | proteins | replication | replication | transcription | transcription | mRNA | mRNA | translation | translation | ribosome | ribosome | nervous system | nervous system | amino acids | amino acids | polypeptide chain | polypeptide chain | cell biology | cell biology | neurobiology | neurobiology | gene regulation | gene regulation | protein structure | protein structure | protein synthesis | protein synthesis | gene structure | gene structure | PCR | PCR | polymerase chain reaction | polymerase chain reaction | protein localization | protein localization | endoplasmic reticulum | endoplasmic reticulum | human biology | human biology | inherited diseases | inherited diseases | developmental biology | developmental biology | evolution | evolution | human genetics | human genetics | human diseases | human diseases | infectious agents | infectious agents | infectious diseases | infectious diseases

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.012 Introduction to Biology (MIT) 7.012 Introduction to Biology (MIT)

Description

All three courses: 7.012, 7.013 and 7.014 cover the same core material which includes: the fundamental principles of biochemistry as they apply to introductory biology, genetics, molecular biology, basic recombinant DNA technology, and gene regulation.In addition, each version of the subject has its own distinctive material, described below. Note: All three versions require a familiarity with some basic chemistry. For details, see the Chemistry Self-evaluation.7.012 focuses on cell biology, immunology, neurobiology, and includes an exploration into current research in cancer, genomics, and molecular medicine. 7.013 focuses on the application of the fundamental principles toward an understanding of cells, human genetics and diseases, infectious agents, cancer, immunology, molecular All three courses: 7.012, 7.013 and 7.014 cover the same core material which includes: the fundamental principles of biochemistry as they apply to introductory biology, genetics, molecular biology, basic recombinant DNA technology, and gene regulation.In addition, each version of the subject has its own distinctive material, described below. Note: All three versions require a familiarity with some basic chemistry. For details, see the Chemistry Self-evaluation.7.012 focuses on cell biology, immunology, neurobiology, and includes an exploration into current research in cancer, genomics, and molecular medicine. 7.013 focuses on the application of the fundamental principles toward an understanding of cells, human genetics and diseases, infectious agents, cancer, immunology, molecular

Subjects

amino acids | amino acids | biochemistry | biochemistry | cancer | cancer | cell biology | cell biology | cell cycle | cell cycle | cell signaling | cell signaling | cloning | cloning | DNA | DNA | endoplasmic reticulum | endoplasmic reticulum | gene regulation | gene regulation | gene structure | gene structure | genetics | genetics | genomics | genomics | immunology | immunology | molecular biology | molecular biology | molecular medicine | molecular medicine | mRNA | mRNA | nervous system | nervous system | neurobiology | neurobiology | PCR | PCR | polymerase chain reaction | polymerase chain reaction | polypeptide chain | polypeptide chain | protein localization | protein localization | protein structure | protein structure | protein synthesis | protein synthesis | proteins | proteins | recombinant DNA | recombinant DNA | replication | replication | ribosome | ribosome | RNA | RNA | stem cells | stem cells | transcription | transcription | translation | translation | virology | virology | biology | biology

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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17.462 Innovation in Military Organizations (MIT) 17.462 Innovation in Military Organizations (MIT)

Description

This seminar has three purposes. One, it inquires into the causes of military innovation by examining a number of the most outstanding historical cases. Two, it views military innovations through the lens of organization theory to develop generalizations about the innovation process within militaries. Three, it uses the empirical study of military innovations as a way to examine the strength and credibility of hypotheses that organization theorists have generated about innovation in non-military organizations. This seminar has three purposes. One, it inquires into the causes of military innovation by examining a number of the most outstanding historical cases. Two, it views military innovations through the lens of organization theory to develop generalizations about the innovation process within militaries. Three, it uses the empirical study of military innovations as a way to examine the strength and credibility of hypotheses that organization theorists have generated about innovation in non-military organizations.

Subjects

URIECA | URIECA | laboratory | laboratory | kinase | kinase | cancer cells | cancer cells | laboratory techniques | laboratory techniques | DNA | DNA | cultures | cultures | UV-Vis | UV-Vis | agarose gel | agarose gel | Abl-gleevec | Abl-gleevec | affinity tags | affinity tags | lyse | lyse | digest | digest | mutants | mutants | resistance | resistance | gel electrophoresis | gel electrophoresis | recombinant | recombinant | nickel affinity | nickel affinity | inhibitors | inhibitors | biochemistry | biochemistry | kinetics | kinetics | enzyme | enzyme | inhibition | inhibition | purification | purification | expression | expression | Political science | Political science | security studies | security studies | innovation | innovation | military organizations | military organizations | war | war | history | history | organization theory | organization theory | empirical study | empirical study | land warfare | land warfare | battleships | battleships | airpower | airpower | submarines | submarines | cruise | cruise | ballistic | ballistic | missiles | missiles | armor | armor | military affairs | military affairs | strategic | strategic | tactical | tactical | counterinsurgency | counterinsurgency | Vietnam | Vietnam | Revolution in Military Affairs | Revolution in Military Affairs | RMA | RMA

License

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20.020 Introduction to Biological Engineering Design (MIT) 20.020 Introduction to Biological Engineering Design (MIT)

Description

Includes audio/video content: AV special element video. This class is a project-based introduction to the engineering of synthetic biological systems. Throughout the term, students develop projects that are responsive to real-world problems of their choosing, and whose solutions depend on biological technologies. Lectures, discussions, and studio exercises will introduce (1) components and control of prokaryotic and eukaryotic behavior, (2) DNA synthesis, standards, and abstraction in biological engineering, and (3) issues of human practice, including biological safety; security; ownership, sharing, and innovation; and ethics. Enrollment preference is given to freshmen. This subject was originally developed and first taught in Spring 2008 by Drew Endy and Natalie Kuldell. Many of Drew's Includes audio/video content: AV special element video. This class is a project-based introduction to the engineering of synthetic biological systems. Throughout the term, students develop projects that are responsive to real-world problems of their choosing, and whose solutions depend on biological technologies. Lectures, discussions, and studio exercises will introduce (1) components and control of prokaryotic and eukaryotic behavior, (2) DNA synthesis, standards, and abstraction in biological engineering, and (3) issues of human practice, including biological safety; security; ownership, sharing, and innovation; and ethics. Enrollment preference is given to freshmen. This subject was originally developed and first taught in Spring 2008 by Drew Endy and Natalie Kuldell. Many of Drew's

Subjects

biology | biology | chemistry | chemistry | synthetic biology | synthetic biology | project | project | biotech | biotech | genetic engineering | genetic engineering | GMO | GMO | ethics | ethics | biomedical ethics | biomedical ethics | genetics | genetics | recombinant DNA | recombinant DNA | DNA | DNA | gene sequencing | gene sequencing | gene synthesis | gene synthesis | biohacking | biohacking | computational biology | computational biology | iGEM | iGEM | BioBrick | BioBrick | systems biology | systems biology

License

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7.013 Introductory Biology (MIT) 7.013 Introductory Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material. 7.013 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer), The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material. 7.013 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer),

Subjects

biology | biology | biochemistry | biochemistry | genetics | genetics | molecular biology | molecular biology | recombinant DNA | recombinant DNA | cell cycle | cell cycle | cell signaling | cell signaling | cloning | cloning | stem cells | stem cells | cancer | cancer | immunology | immunology | virology | virology | genomics | genomics | molecular medicine | molecular medicine | DNA | DNA | RNA | RNA | proteins | proteins | replication | replication | transcription | transcription | mRNA | mRNA | translation | translation | ribosome | ribosome | nervous system | nervous system | amino acids | amino acids | polypeptide chain | polypeptide chain | cell biology | cell biology | neurobiology | neurobiology | gene regulation | gene regulation | protein structure | protein structure | protein synthesis | protein synthesis | gene structure | gene structure | PCR | PCR | polymerase chain reaction | polymerase chain reaction | protein localization | protein localization | endoplasmic reticulum | endoplasmic reticulum | human biology | human biology | inherited diseases | inherited diseases | developmental biology | developmental biology | evolution | evolution | human genetics | human genetics | human diseases | human diseases | infectious agents | infectious agents | infectious diseases | infectious diseases

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.014 Introductory Biology (MIT) 7.014 Introductory Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.014 focuses on the application of these fundamental principles, toward an understanding of microorganisms as geochemical agents responsible for the evolution and renewal of the biosphere and of their role in human health The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.014 focuses on the application of these fundamental principles, toward an understanding of microorganisms as geochemical agents responsible for the evolution and renewal of the biosphere and of their role in human health

Subjects

microorganisms | microorganisms | geochemistry | geochemistry | geochemical agents | geochemical agents | biosphere | biosphere | bacterial genetics | bacterial genetics | carbon metabolism | carbon metabolism | energy metabolism | energy metabolism | productivity | productivity | biogeochemical cycles | biogeochemical cycles | molecular evolution | molecular evolution | population genetics | population genetics | evolution | evolution | population growth | population growth | biology | biology | biochemistry | biochemistry | genetics | genetics | molecular biology | molecular biology | recombinant DNA | recombinant DNA | cell cycle | cell cycle | cell signaling | cell signaling | cloning | cloning | stem cells | stem cells | cancer | cancer | immunology | immunology | virology | virology | genomics | genomics | molecular medicine | molecular medicine | DNA | DNA | RNA | RNA | proteins | proteins | replication | replication | transcription | transcription | mRNA | mRNA | translation | translation | ribosome | ribosome | nervous system | nervous system | amino acids | amino acids | polypeptide chain | polypeptide chain | cell biology | cell biology | neurobiology | neurobiology | gene regulation | gene regulation | protein structure | protein structure | protein synthesis | protein synthesis | gene structure | gene structure | PCR | PCR | polymerase chain reaction | polymerase chain reaction | protein localization | protein localization | endoplasmic reticulum | endoplasmic reticulum | ecology | ecology | communities | communities

License

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Communication (MIT) Communication (MIT)

Description

This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop

Subjects

experimental biology | experimental biology | microbial genetics | microbial genetics | protein biochemistry | protein biochemistry | recombinant DNA | recombinant DNA | development | development | zebrafish | zebrafish | phase contrast microscopy | phase contrast microscopy | teratogenesis | teratogenesis | rna isolation | rna isolation | northern blot | northern blot | gene expression | gene expression | western blot | western blot | PCR | PCR | polymerase chain reaction | polymerase chain reaction | RNA gel | RNA gel | RNA fixation | RNA fixation | probe labeling | probe labeling | mutagenesis | mutagenesis | transposon | transposon | column chromatography | column chromatography | size-exclusion chromatography | size-exclusion chromatography | anion exchange chromatography | anion exchange chromatography | SDS-Page gel | SDS-Page gel | enzyme kinetics | enzyme kinetics | transformation | transformation | primers | primers

License

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7.012 Introduction to Biology (MIT) 7.012 Introduction to Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.AcknowledgmentsThe study materials, problem sets, and quiz materials used during Fall 2004 for The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.AcknowledgmentsThe study materials, problem sets, and quiz materials used during Fall 2004 for

Subjects

biology | biology | biochemistry | biochemistry | genetics | genetics | molecular biology | molecular biology | recombinant DNA | recombinant DNA | cell cycle | cell cycle | cell signaling | cell signaling | cloning | cloning | stem cells | stem cells | cancer | cancer | immunology | immunology | virology | virology | genomics | genomics | molecular medicine | molecular medicine | DNA | DNA | RNA | RNA | proteins | proteins | replication | replication | transcription | transcription | mRNA | mRNA | translation | translation | ribosome | ribosome | nervous system | nervous system | amino acids | amino acids | polypeptide chain | polypeptide chain | cell biology | cell biology | neurobiology | neurobiology | gene regulation | gene regulation | protein structure | protein structure | protein synthesis | protein synthesis | gene structure | gene structure | PCR | PCR | polymerase chain reaction | polymerase chain reaction | protein localization | protein localization | endoplasmic reticulum | endoplasmic reticulum

License

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2.72 Elements of Mechanical Design (MIT) 2.72 Elements of Mechanical Design (MIT)

Description

This is an advanced course on modeling, design, integration and best practices for use of machine elements such as bearings, springs, gears, cams and mechanisms. Modeling and analysis of these elements is based upon extensive application of physics, mathematics and core mechanical engineering principles (solid mechanics, fluid mechanics, manufacturing, estimation, computer simulation, etc.). These principles are reinforced via (1) hands-on laboratory experiences wherein students conduct experiments and disassemble machines and (2) a substantial design project wherein students model, design, fabricate and characterize a mechanical system that is relevant to a real world application. Students master the materials via problems sets that are directly related to, and coordinated with, the deliv This is an advanced course on modeling, design, integration and best practices for use of machine elements such as bearings, springs, gears, cams and mechanisms. Modeling and analysis of these elements is based upon extensive application of physics, mathematics and core mechanical engineering principles (solid mechanics, fluid mechanics, manufacturing, estimation, computer simulation, etc.). These principles are reinforced via (1) hands-on laboratory experiences wherein students conduct experiments and disassemble machines and (2) a substantial design project wherein students model, design, fabricate and characterize a mechanical system that is relevant to a real world application. Students master the materials via problems sets that are directly related to, and coordinated with, the deliv

Subjects

biology | biology | chemistry | chemistry | synthetic biology | synthetic biology | project | project | biotech | biotech | genetic engineering | genetic engineering | GMO | GMO | ethics | ethics | biomedical ethics | biomedical ethics | genetics | genetics | recombinant DNA | recombinant DNA | DNA | DNA | gene sequencing | gene sequencing | gene synthesis | gene synthesis | biohacking | biohacking | computational biology | computational biology | iGEM | iGEM | BioBrick | BioBrick | systems biology | systems biology | machine design | machine design | hardware | hardware | machine element | machine element | design process | design process | design layout | design layout | prototype | prototype | mechanism | mechanism | engineering | engineering | fabrication | fabrication | lathe | lathe | precision engineering | precision engineering | group project | group project | project management | project management | CAD | CAD | fatigue | fatigue | Gantt chart | Gantt chart

License

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5.36 Biochemistry Laboratory (MIT) 5.36 Biochemistry Laboratory (MIT)

Description

The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing. This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format. Acknowledgments Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan. The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing. This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format. Acknowledgments Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan.

Subjects

URIECA | URIECA | laboratory | laboratory | kinase | kinase | cancer cells | cancer cells | laboratory techniques | laboratory techniques | DNA | DNA | cultures | cultures | UV-Vis | UV-Vis | agarose gel | agarose gel | Abl-gleevec | Abl-gleevec | affinity tags | affinity tags | lyse | lyse | digest | digest | mutants | mutants | resistance | resistance | gel electrophoresis | gel electrophoresis | recombinant | recombinant | nickel affinity | nickel affinity | inhibitors | inhibitors | biochemistry | biochemistry | kinetics | kinetics | enzyme | enzyme | inhibition | inhibition | purification | purification | expression | expression

License

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7.013 Introductory Biology (MIT) 7.013 Introductory Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. 7.013 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer), developmental biology, neurobiology and evolution.Biological function at the molecular level is particularly emphasized in all courses and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In add The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. 7.013 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer), developmental biology, neurobiology and evolution.Biological function at the molecular level is particularly emphasized in all courses and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In add

Subjects

biology | biology | biochemistry | biochemistry | genetics | genetics | molecular biology | molecular biology | recombinant DNA | recombinant DNA | cell cycle | cell cycle | cell signaling | cell signaling | cloning | cloning | stem cells | stem cells | cancer | cancer | immunology | immunology | virology | virology | genomics | genomics | molecular medicine | molecular medicine | DNA | DNA | RNA | RNA | proteins | proteins | replication | replication | transcription | transcription | mRNA | mRNA | translation | translation | ribosome | ribosome | nervous system | nervous system | amino acids | amino acids | polypeptide chain | polypeptide chain | cell biology | cell biology | neurobiology | neurobiology | gene regulation | gene regulation | protein structure | protein structure | protein synthesis | protein synthesis | gene structure | gene structure | PCR | PCR | polymerase chain reaction | polymerase chain reaction | protein localization | protein localization | endoplasmic reticulum | endoplasmic reticulum | human biology | human biology | inherited diseases | inherited diseases | developmental biology | developmental biology | evolution | evolution | human genetics | human genetics | human diseases | human diseases | infectious agents | infectious agents | infectious diseases | infectious diseases

License

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7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT) 7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT)

Description

One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a

Subjects

Green Fluorescent Protein | Green Fluorescent Protein | Fluorescent protein engineering | Fluorescent protein engineering | Photoconversion | Photoconversion | fluorescent protein variants | fluorescent protein variants | fluorescent microscopy facility | fluorescent microscopy facility | Quantitative fluorescent imaging | Quantitative fluorescent imaging | ultra-sensitive fluorescent imaging | ultra-sensitive fluorescent imaging | high-throughput analysis | high-throughput analysis | Fluorescent imaging in living organisms | Fluorescent imaging in living organisms | phycoerythrin | phycoerythrin | phytochrome | phytochrome | jellyfish | jellyfish | red fluorescent protein | red fluorescent protein | photoactivation | photoactivation | chromophore | chromophore | protonation | protonation | lysosomes | lysosomes | recombinant protein molecules | recombinant protein molecules

License

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STS.014 Principles and Practice of Science Communication (MIT) STS.014 Principles and Practice of Science Communication (MIT)

Description

This course helps in developing skills as science communicators through projects and analysis of theoretical principles. Case studies explore the emergence of popular science communication over the past two centuries and consider the relationships among authors, audiences and media. Project topics are identified early in the term and students work with MIT Museum staff. Projects may include physical exhibits, practical demonstrations, or scripts for public programs. This course helps in developing skills as science communicators through projects and analysis of theoretical principles. Case studies explore the emergence of popular science communication over the past two centuries and consider the relationships among authors, audiences and media. Project topics are identified early in the term and students work with MIT Museum staff. Projects may include physical exhibits, practical demonstrations, or scripts for public programs.

Subjects

public understanding of science | public understanding of science | science writing | science writing | museum | museum | exhibit | exhibit | debate | debate | journalism | journalism | stem cell | stem cell | recombinant DNA | recombinant DNA | intelligent design | intelligent design | GMA | GMA | genetically modified food | genetically modified food | biotechnology | biotechnology | bioengineering | bioengineering | risk | risk | journal | journal | newspaper | newspaper | radio | radio | fraud | fraud | cloning | cloning | evolution | evolution | controversy | controversy

License

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Malaria vaccine for P. vivax

Description

Dr Arturo Reyes-Sandoval tells us about his research on a vaccine against Plasmodium vivax. Dr Reyes Sandoval aims to develop a novel malaria vaccine against Plasmodium vivax, one of the four malaria parasites that affect humans. P. vivax is found in Africa, Asia, Latin America and the Western Pacific. 40 percent of the world's population is exposed to the disease that is responsible for around 130 to 350 million clinical cases every year. Wales; http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

Subjects

recombinant viral vectors | Plasmodium vivax | malaria vaccines | recombinant viral vectors | Plasmodium vivax | malaria vaccines

License

http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

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Malaria vaccine for P. vivax

Description

Dr Arturo Reyes-Sandoval tells us about his research on a vaccine against Plasmodium vivax. Dr Reyes Sandoval aims to develop a novel malaria vaccine against Plasmodium vivax, one of the four malaria parasites that affect humans. P. vivax is found in Africa, Asia, Latin America and the Western Pacific. 40 percent of the world's population is exposed to the disease that is responsible for around 130 to 350 million clinical cases every year. Wales; http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

Subjects

recombinant viral vectors | Plasmodium vivax | malaria vaccines | recombinant viral vectors | Plasmodium vivax | malaria vaccines

License

http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

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7.016 Introductory Biology (MIT) 7.016 Introductory Biology (MIT)

Description

7.016 Introductory Biology provides an introduction to fundamental principles of biochemistry, molecular biology and genetics for understanding the functions of living systems. Taught for the first time in Fall 2013, this course covers examples of the use of chemical biology and twenty-first-century molecular genetics in understanding human health and therapeutic intervention. The MIT Biology Department Introductory Biology courses, 7.012, 7.013, 7.014, 7.015, and 7.016 all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as the structure and synthesis of proteins, how these mol 7.016 Introductory Biology provides an introduction to fundamental principles of biochemistry, molecular biology and genetics for understanding the functions of living systems. Taught for the first time in Fall 2013, this course covers examples of the use of chemical biology and twenty-first-century molecular genetics in understanding human health and therapeutic intervention. The MIT Biology Department Introductory Biology courses, 7.012, 7.013, 7.014, 7.015, and 7.016 all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as the structure and synthesis of proteins, how these mol

Subjects

biochemistry | biochemistry | molecular biology | molecular biology | genetics | genetics | human genetics | human genetics | pedigrees | pedigrees | biochemical genetics | biochemical genetics | chemical biology | chemical biology | molecular genetics | molecular genetics | recombinant DNA technology | recombinant DNA technology | cell biology | cell biology | cancer | cancer | viruses | viruses | HIV | HIV | bacteria | bacteria | antibiotics | antibiotics | human health | human health | therapeutic intervention | therapeutic intervention | cell signaling | cell signaling | evolution | evolution | reproduction | reproduction | infectious diseases | infectious diseases | therapeutics | therapeutics

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm

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7.01SC Fundamentals of Biology (MIT)

Description

Fundamentals of Biology focuses on the basic principles of biochemistry, molecular biology, genetics, and recombinant DNA. These principles are necessary to understanding the basic mechanisms of life and anchor the biological knowledge that is required to understand many of the challenges in everyday life, from human health and disease to loss of biodiversity and environmental quality.

Subjects

amino acids | carboxyl group | amino group | side chains | polar | hydrophobic | primary structure | secondary structure | tertiary structure | quaternary structure | x-ray crystallography | alpha helix | beta sheet | ionic bond | non-polar bond | van der Waals interactions | proton gradient | cyclic photophosphorylation | sunlight | ATP | chlorophyll | chlorophyll a | electrons | hydrogen sulfide | biosynthesis | non-cyclic photophosphorylation | photosystem II | photosystem I | cyanobacteria | chloroplast | stroma | thylakoid membrane | Genetics | Mendel | Mendel's Laws | cloning | restriction enzymes | vector | insert DNA | ligase | library | E.Coli | phosphatase | yeast | transformation | ARG1 gene | ARG1 mutant yeast | yeast wild-type | cloning by complementation | Human Beta Globin gene | protein tetramer | vectors | antibodies | human promoter | splicing | mRNA | cDNA | reverse transcriptase | plasmid | electrophoresis | DNA sequencing | primer | template | capillary tube | laser detector | human genome project | recombinant DNA | clone | primer walking | subcloning | computer assembly | shotgun sequencing | open reading frame | databases | polymerase chain reaction (PCR) | polymerase | nucleotides | Thermus aquaticus | Taq polymerase | thermocycler | resequencing | in vitro fertilization | pre-implantation diagnostics | forensics | genetic engineering | DNA sequences | therapeutic proteins | E. coli | disease-causing mutations | cleavage of DNA | bacterial transformation | recombinant DNA revolution | biotechnology industry | Robert Swanson | toxin gene | pathogenic bacterium | biomedical research | S. Pyogenes | origin of replication

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.014 Introductory Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.014 focuses on the application of these fundamental principles, toward an understanding of microorganisms as geochemical agents responsible for the evolution and renewal of the biosphere and of their role in human health

Subjects

microorganisms | geochemistry | geochemical agents | biosphere | bacterial genetics | carbon metabolism | energy metabolism | productivity | biogeochemical cycles | molecular evolution | population genetics | evolution | population growth | biology | biochemistry | genetics | molecular biology | recombinant DNA | cell cycle | cell signaling | cloning | stem cells | cancer | immunology | virology | genomics | molecular medicine | DNA | RNA | proteins | replication | transcription | mRNA | translation | ribosome | nervous system | amino acids | polypeptide chain | cell biology | neurobiology | gene regulation | protein structure | protein synthesis | gene structure | PCR | polymerase chain reaction | protein localization | endoplasmic reticulum | ecology | communities

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.012 Introduction to Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.AcknowledgmentsThe study materials, problem sets, and quiz materials used during Fall 2004 for

Subjects

biology | biochemistry | genetics | molecular biology | recombinant DNA | cell cycle | cell signaling | cloning | stem cells | cancer | immunology | virology | genomics | molecular medicine | DNA | RNA | proteins | replication | transcription | mRNA | translation | ribosome | nervous system | amino acids | polypeptide chain | cell biology | neurobiology | gene regulation | protein structure | protein synthesis | gene structure | PCR | polymerase chain reaction | protein localization | endoplasmic reticulum

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.012 Introduction to Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.AcknowledgmentsThe study materials, problem sets, and quiz materials used during Fall 2004 for

Subjects

biology | biochemistry | genetics | molecular biology | recombinant DNA | cell cycle | cell signaling | cloning | stem cells | cancer | immunology | virology | genomics | molecular medicine | DNA | RNA | proteins | replication | transcription | mRNA | translation | ribosome | nervous system | amino acids | polypeptide chain | cell biology | neurobiology | gene regulation | protein structure | protein synthesis | gene structure | PCR | polymerase chain reaction | protein localization | endoplasmic reticulum

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.341 Brightening up Life: Harnessing the Power of Fluorescence Imaging to Observe Biology in Action (MIT)

Description

One summer in the 1960s a young Japanese researcher, with the help of a few high school students, chopped up ten thousand jellyfish. As a by-product of this harvest, they isolated a green fluorescent protein (GFP). Since then, GFP has triggered a revolution in our understanding of gene expression and signaling in live cells. In this seminar, we will examine how this small protein generates fluorescence, i.e. absorbs light of one wavelength and emits light of a longer wavelength. We will discuss how the color palette has been extended from green to blue, red and many other colors, based on protein engineering of GFP and the study of vividly colorful coral reefs. We will then investigate how these fluorescent proteins can be used to track the motion of DNA, RNA and protein in living cells, a

Subjects

Green Fluorescent Protein | Fluorescent protein engineering | Photoconversion | fluorescent protein variants | fluorescent microscopy facility | Quantitative fluorescent imaging | ultra-sensitive fluorescent imaging | high-throughput analysis | Fluorescent imaging in living organisms | phycoerythrin | phytochrome | jellyfish | red fluorescent protein | photoactivation | chromophore | protonation | lysosomes | recombinant protein molecules

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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Communication (MIT)

Description

This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research projects. Development of skills critical for writing about scientific findings in modern biology is also covered in the Scientific Communications portion of the curriculum, 7.02CI. Additional Faculty Dr. Katherine Bacon Schneider Dr. Jean-Francois Hamel Ms. Deborah Kruzel Dr. Megan Rokop

Subjects

experimental biology | microbial genetics | protein biochemistry | recombinant DNA | development | zebrafish | phase contrast microscopy | teratogenesis | rna isolation | northern blot | gene expression | western blot | PCR | polymerase chain reaction | RNA gel | RNA fixation | probe labeling | mutagenesis | transposon | column chromatography | size-exclusion chromatography | anion exchange chromatography | SDS-Page gel | enzyme kinetics | transformation | primers

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.012 Introduction to Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.AcknowledgmentsThe study materials, problem sets, and quiz materials used during Fall 2004 for

Subjects

biology | biochemistry | genetics | molecular biology | recombinant DNA | cell cycle | cell signaling | cloning | stem cells | cancer | immunology | virology | genomics | molecular medicine | DNA | RNA | proteins | replication | transcription | mRNA | translation | ribosome | nervous system | amino acids | polypeptide chain | cell biology | neurobiology | gene regulation | protein structure | protein synthesis | gene structure | PCR | polymerase chain reaction | protein localization | endoplasmic reticulum

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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7.012 Introduction to Biology (MIT)

Description

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.AcknowledgmentsThe study materials, problem sets, and quiz materials used during Fall 2004 for

Subjects

biology | biochemistry | genetics | molecular biology | recombinant DNA | cell cycle | cell signaling | cloning | stem cells | cancer | immunology | virology | genomics | molecular medicine | DNA | RNA | proteins | replication | transcription | mRNA | translation | ribosome | nervous system | amino acids | polypeptide chain | cell biology | neurobiology | gene regulation | protein structure | protein synthesis | gene structure | PCR | polymerase chain reaction | protein localization | endoplasmic reticulum

License

Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see https://ocw.mit.edu/terms/index.htm

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